Biological Testing, Including Pathogens


Marshfield Food Safety works with companies to conduct microbiological and chemistry testing.  Food testing is required to obtain a certificate of analysis for ready to eat and raw products at certain stages of processing.  Customers are provided analyses to meet requirements for Food Process Evaluation.

Having A2LA Accreditation to ISO 17025:2005 assures our results will make you compliant with USDA FSIS, HACCP, US FDA FSMA, and other regulatory requirements.  To search for a specific test you may view our food testing menu or contact us to inquire.  Food testing technologies are listed below.

Virtual Tour Video of Marshfield Food Testing Laboratory

Services include but are not limited to

Environmental Monitoring
Quality System Audits 
Check Sample Programs 
Process Control Chemistries 
Other Process Control Parameter Measurements


Polymerase chain-reaction (PCR) testing identifies major pathogens like E.coli 0157:H7, Listeria monocytogenes, Salmonella and Campylobacter by detecting the organism's DNA. This extremely rapid, accurate testing technology can detect as few as one bacterial organism per sample in as little as eight hours. PCR is several hours faster than standard tests now used in the food industry. Extremely rapid product testing for the major food pathogens using this technology will increase the food safety protection for the food industry and consumer.

Enzyme-Linked Fluorescent Assay (ELFA) reveals pathogens by detecting their protein. This technology is currently the cheapest and most widely used in the food industry.

Cultures from food sources are grown on plates and visually examined for pathogenic species. This is the time-tested method of detection. Organisms can also be isolated and/or quantified using plate counts or MPN methods. 

Shelf-Life Verification
Shelf-life Verification allows clients to maximize stability of product and apply it to competitive pricing. Microorganism Strain Identification by Pulse-Field Gel Electrophoresis aids identification of spoilage organisms.  Through strain identification, DNA is abstracted, multiplied and mapped to isolate certain strands or unique variances that could be causing problems in stability.  

Molecular Pathogen Detection
The Atlas System is a fully automated instrument that enhances the accuracy, speed, and efficiency of food safety testing through detection of molecular pathogens, including Listeria, SalmonellaEscherichia coli, and other organisms commonly implicated in food contamination episodes.
Cutting Edge Pathogen Detection with Roka Atlas


The state-of-the-art, fully automated Roka Atlas Systemincreases the accuracy, speed, and efficiency of our food safety testing processes.


The system accurately detects molecular pathogens commonly implicated in food contamination, including:

  • E. coli O157:H7
  • E. coli STEC
  • Listeria
  • Salmonella

This fully validated and proven technology is FSIS compliant and A2LA accredited.

Validation Study has been completed to show Atlas STEC EG2 Combo Detection Assay for Big Six Non-O157 Shiga Toxin Producing Escherichia coli (STEC) in Ground Beef, Beef Trim, andFrozen Beef (FTB). 

Salmonella Limits Testing in Poultry

The Roka Salmonella assay uses a shortened enrichment protocol taking 4 hours, then transfer to lysis tubes, followed by 3 hour analysis time on the Atlas instrument.  This is possible because the Atlas instrument provides more sensitivity for detection, using rRNA as target instead of DNA.  Follow this link to see more details at the Roka Science Blog.

Contact us to Request Quote:
Hondo Dammann
Client Services Field Technologist
888-780-9897, ext 1

Food Testing Lab Accredited
to ISO/IEC 17025:2005

A2LA Accredited

Testing Certificate Numbers

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